Flow cytometry service

Applications

• Multi-parameter analysis of both extra and intracellular labelling for cellular immunophenotyping and functionality.

• Gene expression analysis using reporter genes.

• Measure of cell proliferation: CFDA, BrdU, CFSE, etc.

• Cell cycle: Cyclins, DNA content

• Apoptosis assays (plasma membrane integrity, mitochondrial function, caspase activity, DNA fragmentation, etc.)

• Cytokine secretion by multiplex assay

• FRET.

• Separation of cell subpopulations based on the differential expression of various intra and extracellular markers.

Ongoing Applications

• Protein localization using PulSA methodology.

• Development of new cell separation applications by cell sorting.

• Separation of large particles, as yeast clusters or small larvae of C. Elegans.

• Separation of small particles, below 1um based on their phenotypic characteristics eg microvesicles.

• High dimensional data analysis , such as dimensionality reduction and clustering algorithms using Cytobank.

Tecnical Support

• New User Equipment Management Course.

• General monitoring and maintenance of analysers.

• Help users with equipment problems, whether technical or experimental.

• Support on experimental design and methodology.