Histology

Contact details

Principal Investigator

Enrique de Álava CasadoEnrique de Álava Casado

Technician in Charge

Rocío Durán RomeroRocío Durán Romero

Histology

  1. Introduction
  2. Techniques
  3. Equipment
  4. Usage rules
  5. Prices
Histology

The institute is provided with a Histology Service with equipment for processing, cutting and staining biological samples.

The objectives of this service are:

  • Maintenance and coordination of the service’ equipment.
  • Training on specialized techniques developed by the specialist.
  • Mandatory training for users in self-service mode.
  • Devwelopment of experimental procedures by the specialist.

Location:1st and 2nd Floor

Working hours:M-F: From 9 a.m. to 5 p.m. *

(*) Those experiments that need time past the established hours could be made prior knowledge of the technician in charge.

The activities that can be performed in the laboratory are, among others:

  • Cytochemical and immunohistochemical techniques.
  • Perfusion of animals.
  • Sample processing in paraffin.
  • Processing and control of the automatic tissue processor.
    • Descaling samples.
    • Full processing paraffin embedding.
    • Serial cut on microtome and tissue assembly.
    • Dewaxing cuts, staining performing.
    • Dehydration and slide assembly.
  • Frozen samples processing.
    • Preparation of frozen blocks.
    • Serial sections of frozen blocks and cuts collection in slide or flotation.
    • Staining of frozen sections.
    • Sample preparation for cutting vibratome.
    • Serial cuts of samples in vibratome and assembling.
  • Stains.
    • Hematoxylin-eosin.
    • Masson trichrome.
    • Nissl.

Specific staining protocols could be developed on demand.

Click on the different equipment for details on features.

Magnetic Stirrer RAYPA AG-2

1ª Planta y 2ª Planta

Magnetic Stirrer RAYPA AG-2

Orbital Shaker VWR

1ª Planta y 2ª Planta

Orbital Shaker VWR

Waterbath LEICA HI1210

Es un baño de agua con una superficie que proporciona una alta conductividad térmica  y excelente resistencia al rayado  debido a su recubrimiento de platico especial.

Las  temperaturas pueden ser seleccionadas entre la temperatura ambiente y 75º.

El borde amplio permite el conveniente almacenamiento de los portaobjetos.

 

[1ª Planta]

Waterbath LEICA HI1210

Waterbath LABOLAN

Baño de agua para las secciones de parafina. Es un baño de agua con una superficie que proporciona una alta conductividad térmica  y excelente resistencia al rayado  debido a su recubrimiento de platico especial.

Las  temperaturas pueden ser seleccionadas entre la temperatura ambiente y 75º.

El borde amplio permite el conveniente almacenamiento de los portaobjetos.

 

[2ª Planta]

Waterbath LABOLAN

Thermostatic Baths RAYPA ECOBATH

1ª Planta y 2ª Planta

Thermostatic Baths RAYPA ECOBATH

Peristaltic pumpus Thermo SCIENTIFIC FH100

1ª Planta y 2ª Planta

Extraction Hoods alcohols battery

1ª Planta y 2ª Planta

Extraction Hoods alcohols battery

LEICA CM1950 Cryostat

Ensure efficient processing of samples, providing at the same time fast and safe cuts.

Equipment providing top quality cuts, due to a high precision microtome with sample progress through the motor controlled step by step.

It also includes powered cutting (optional).

To increase the security inside the machine, it has a UVC disinfection system to remove bacteria, viruses and fungus even at low temperatures.

Waste removal system and spacious cryochamber.

[ 1st and 2nd floor ]

LEICA CM1950 Cryostat

Paraffin dispenser LEICA EG1122

La parafina se dispensa a través de boquillas dispensadoras con calefacción  y el caudal se puede regular fácilmente según sea necesario para garantizar la disensión de parafina sin problemas, en todo tipo de situaciones de trabajo.

 

[1ª Planta y 2ª Planta]

Paraffin dispenser LEICA EG1122

Bacteriological and culture ovens RAYPA

1ª Planta y 2ª Planta

Bacteriological and culture ovens  RAYPA

Transmitted light magnifiers OLYMPUS SZ7

1ª Planta y 2º Planta

Transmitted light magnifiers OLYMPUS SZ7

Stereomicroscope system OLYMPUS SZX16

1ª Planta y 2ª Planta

Stereomicroscope system OLYMPUS SZX16

LEICA RM2255 Motorized Microtome (paraffin)

The fully automated rotary microtome Leica RM2255, designed for paraffin samples sectioning, offers a wide range of applications for histology research in laboratories. The RM2255 is designed for customers who want to integrate samples in paraffin or resin in clinical pathology and biomedical research. It is fully powered by a stepper motor, with a precision orientation system and a large powerful engine for cutting. The microtome can be operated manually too.

The cutting speed can be adjusted to cut hard materials and paraffin embedded samples with high performance without detriment to the sectioning quality.

Its features are: fully motorized rotary microtome with adjustable cutting speed and two speeds of movement, ergonomic design with an external control panel, selection of steps or continuous motion, safety lever with two locking mechanisms, integrated storage tray at the top of the microtome, internal mechanism protection system, specimen clamp system, one-handed operation way and integrated section waste tray.

[ 1st and 2nd Floor ]

Cold Plate LEICA EG 1150C

La placa fría se mantiene a una temperatura constante de – 5ºC,  proporcionando un rápido enfriamiento de los bloques.

La gran capacidad de la placa puede contener más de 70 moldes estándar de cassettes.

La placa fría se puede utilizar como una unidad independiente para enfriar los bloques antes de seccionar o como centro de incrustación para enfriar moldes después de la dispensión de parafina.

 

[1ª Planta y 2ª Planta]

Cold Plate LEICA EG 1150C

Flattening table LEICA HI1210

La placa calefactora Leica  tiene una gran superficie de trabajo  de aluminio negro proporcionando una alta conductividad térmica.

Las  temperaturas pueden ser seleccionadas entre la temperatura ambiente y 75º.

 

[1ª Planta y 2ª Planta]

Flattening table LEICA  HI1210

LEICA ASP300S Automated tissue processor

Modular design equipment with the following applications: Fixation, dehydration, and infiltration in paraffin of tissue samples.

The processing unit is comprised of three paraffin stations and the retort.

There are tree baskets for cassettes each with a capacity of 100 cassettes.

The processor is programmed via a color touch screen, LCP type.

The control software gives detailed information on all features of the display.

[ 1st floor ]

Sackers and Rockers STUAR SSM4

1ª Planta y 2ª Planta

Sackers and Rockers STUAR SSM4

LEICA 1200S Microtome with Vibrating Blade

Equipment using a vibrating blade that allows precise sectioning without freezing or embedding, so that fresh tissue viability can be maintained properly.

Adjustable advancement speed of the blade.

Fully automated cutting mode. It has a high rate of return of the blade of 5 mm / s and a programmable cutting window that guarantees a extremely fast switching, even in the smallest specimens.

[ 1st floor ]

LEICA 1200S Microtome with Vibrating Blade

LEICA VT1000S Microtome with Vibrating Blade

Equipment using a vibrating blade that allows precise sectioning without freezing or embedding, so that fresh tissue viability can be maintained properly.

Adjustable advancement speed of the blade.

Fully automated cutting mode. It has a high rate of return of the blade of 5 mm / s and a programmable cutting window that guarantees a extremely fast switching, even in the smallest specimens.

[ 1st and 2nd floor ]

LEICA VT1000S Microtome with Vibrating Blade

AS A GENERAL RULES

  • The only histology devices to which users have direct access are: cryostat, microtome and vibratome. They are available from Monday to Sunday, as long as the reservation has been made on time.

Automatic Tissue Processor and Paraffin Dispenser are restricted exclusively to staff.

  • Anyone who wants access to cut equipment must ask previously the responsible technician, who is the only responsible person for training users and authorize the use of the equipments once they are trained.
  • A maximum of 3 hours per user could be booked. If there are not to be used, reservation must be cancelled immediately.
  • Please be punctual when using the equipment. More than 15 minutes of delay or misuses of the equipment would cause the booking cancellation or the impediment of use of the equipment.
  • The equipment must not be switched on at night, unless the responsible technician was aware.
  • The consumables, molds, slides, cassettes, antirolling glasses, knives, etc. will be provided by the user.

To ensure the smooth operation of the service, the service technician is obliged to inform the respective IR in case of repeated breach of any of these rules. IBiS Management Team will take control measures in case of continued misuses.

Below you can download the applicable fees.

For information about fees and charges applicable to our services, please contact: histologia-ibis@us.es.