Cardiovascular and Respiratory Pathology/Other Systemic Diseases

Clinical and molecular epidemiology

Enrique J. Calderón Sandubete
Enrique J. Calderón Sandubete
Campus Hospital Universitario Virgen del Rocío
Avda. Manuel Siurot, s/n.
41013 · Sevilla
Laboratory: 206
Francisco Javier Medrano Ortega
Francisco Javier Medrano Ortega
Campus Hospital Universitario Virgen del Rocío
Avda. Manuel Siurot, s/n.
41013 · Sevilla
Laboratory: 206
Pablo Stiefel García-Junco
Pablo Stiefel García-Junco
Campus Hospital Universitario Virgen del Rocío
Avda. Manuel Siurot, s/n.
41013 · Sevilla

Enrique J. Calderón Sandubete

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  1. Group members
  2. Research areas
  3. Publications

Group: Clinical and molecular epidemiology

Group members Clinical and molecular epidemiology
  • Alfaro Lara, Verónica.Lda. en Medicina y Cirugía. FEA (HUVR)
  • Artero González, María Luisa.Degree in Biology. Technician.
  • Beltrán Romero, Luis Matías.FEA HUVR.
  • Briones Pérez de la Blanca, Eduardo.Doctor en Medicina
  • Calderón Sandubete, Enrique J..MD/PhD. Faculty Staff - Internal Medicine/Assistant Professor (HUVR/US).
  • Delgado de la Cuesta, Juan.Dr. en Medicina y Cirugía
  • Domínguez Simeón, Mª Jesús.Técnico Sup. de Laboratorio de Anatomía Patológica y Citología (FP2). Técnico.
  • Escorial Moya, Cristina.Dra. en Medicina y Cirugía
  • Espinosa Torre, Fátima.Lda. en Medicina. MIR.
  • Fernández Ojeda, María del Rocío.Doctorado en Medicina y Cirugía.
  • Friaza Patiño, Vicente.Degree in Biology & Biochemistry. Technician.
  • García Hernández, Francisco José.MD/PhD. Faculty Staff - Internal Medicine (HUVR).
  • González Estrada, Aurora.Lda. en Medicina y Cirugía. MIR (HUVR) Doctora en Biología Molecular
  • González León, Rocío.MD/PhD. Faculty Staff - Internal Medicine (HUVR).
  • Gutiérrez Rivero, Sonia.Degree in Medicine & Surgery. Faculty Staff - Internal Medicine (HUVR).
  • Horra Padilla, Carmen de la.PhD in Pharmacy. Post-doctoral fellow Miguel Servet.
  • León Jiménez, David.FEA HUVR
  • Martín Garrido, Isabel.FEA HUVR. Doctora en Medicina
  • Mate Barrero, Alfonso.Doctor en Farmacia
  • Medrano Ortega, Francisco Javier.MD/PhD. Faculty Staff - Internal Medicine/Prof. Asociado (HUVR/US).
  • Miranda Guisado, Mª Luisa.Doctora en Medicina. FEA (HUVR).
  • Morilla Romero de la Osa, Rubén.Doctor en Bioquímica. Ayudante Doctor US.
  • Muñiz Grijalvo, Ovidio.Doctor en Medicina. FEA (HUVR).
  • Navarro Alonso, Mar.Técnico
  • Navarro Morán, Arturo Manuel.Diplomado en Enfermería
  • Respaldiza Salas, María Nieves.MD/PhD. Assistant Professor (Pablo de Olavide University).
  • Reyes Goya, Claudia.Doctorado en Biología Molecular.
  • Rivero Rivero, Laura.MD/PhD. Faculty Staff - Internal Medicine (HUVR).
  • Salsoso Rodríguez, Rocío.Doctorado en Ciencias Médicas
  • Santana Garrido, Álvaro.Investigador Predoctoral. Ldo. en Farmacia
  • Stiefel García-Junco, Pablo.Doctor en Medicina y Cirugía. FEA (HUVR).
  • Vallejo Vaz, Antonio Javier.Doctor en Medicina y Cirugía
  • Varela Aguilar, José Manuel.MD/PhD. Faculty Staff - Internal Medicine (HUVR).
  • Vázquez Cueto, Carmen María.Doctora en Farmacia
  • Yang Lai, Rosa María.Degree in Medicine. Faculty Staff - Primary Attention Medicine/Assistant Professor.

Research areas

Our research team is multidisciplinary and includes physicians and basic researchers with training in different areas of the biomedical field. In recent years we have focused our work on two main research areas: (a) The epidemiology and physiopathology of infection by Pneumocystis jirovecii (formerly P. carinii sp. f. hominis), and (b) the development of methods for the evaluation and improvement of clinical practice.

(a) Epidemiology and physiopathology of Pneumocystis jirovecii infection

  • Epidemiology of P. jirovecii. For almost a century Pneumocystis carinii was considered to be a single protozoan, able to invade and proliferate in the lung of deeply immunosuppressed human and non-human mammalian hosts. The impossibility to develop continuous in vitro cultures has limited advances in knowledge about this microorganism. Recently, molecular biology techniques have permitted it to be demonstrated that the term "P. carinii" masks a variety of ubiquitous atypical fungal parasites with strong host specificity that are actual species which are currently being described. To the present time, only one species has been identified in human hosts and has been named Pneumocystis jirovecii. Currently, many characteristics of its biology and epidemiology are unknown. Thus, identification of human-Pneumocystis reservoirs and sources of infection using molecular tools constitutes to us an important area for research.
  • Role of Pneumocystis jirovecii colonization in chronic pulmonary diseases. The development of sensitive molecular techniques has led to the recognition of a colonization or carrier state of Pneumocystis jirovecii, in which low levels of the organism are detected in persons who do not have overt Pneumocystis pneumonia. Pneumocystis colonization has been described in subjects with various chronic lung diseases, and accumulating evidence suggests that it may be an important clinical phenomenon. Unravelling the role of P. jirovecii colonization in the pathophysiology of the most frequent chronic pulmonary diseases (COPD, Cystic Fibrosis, or Idiopathic pulmonary fibrosis) is another important area of research for our group.
  • Biology of Pneumocystis jirovecii. Although still impaired by the lack of a reliable and reproducible continuous in vitro cultivation system, research into the natural history of P. jirovecii has advanced owing to the expansion of molecular-based techniques. However, little is known about the biological characteristics of P. jirovecii, since the lack of culture methods has obliged researchers to use animal models, for which most of the knowledge produced concerns rat-derived Pneumocystis. For this reason, new post-genomic approaches using transcriptomic and proteomic tools are being developed to improve our knowledge about the biology of P. jirovecii.

(b) Clinical practice improvement and evaluation.

  • Development of evidence-based clinical practice guidelines. Our group has developed a CPG for venous thromboembolism prevention in medical patients (PRETEMED), which has been endorsed by different scientific societies and included in the National Guideline Clearinghouse (NGC: 003602). We are currently evaluating different CPG implementation strategies and their effectiveness to change medical practices.
International Journals
Gomez, JMNG; Vega, GPS; Cacicedo, J; Leon, BDD; Rivera, DH; Fernandez, JMP; del Campo, ER; Gordillo, MJO; Guerra, JLL
Impact of pre-radiation therapy quality of life in lung cancer survival: a prospective, intention-to-treat, multicenter study
Panizo, MM; Ferrara, G; Garcia, N; Moreno, X; Navas, T; Calderon, E; Matos, O; de La Horra, C; Friaza, V; Reviakina, V; Dolande, M; Alarcon, V; Caceres, AM
Epidemiology of Pneumocystis jirovecii Pneumonia in Venezuela
Chiarello, DI; Abad, C; Rojas, D; Toledo, F; Vazquez, CM; Mate, A; Sobrevia, L; Marin, R
Oxidative stress: Normal pregnancy versus preeclampsia
Cabanillas, LD; Centeno, MDC; de la Osa, RMR; Sandubete, EJC
Cross-sectional study of the healthcare provided by a medicalized helicopter from Extremadura (Spain)
Leon-Jimenez, D; Moreno-Obregon, F; Beltran-Romero, LM; Miramontes-Gonzalez, JP
Is the joint use of diuretics and SGLT2 inhibitors beneficial in diabetic kidney disease?
Santana-Garrido, A; Reyes-Goya, C; Andre, H; Aramburu, O; Mate, A; Vazquez, CM
Sunitinib-induced oxidative imbalance and retinotoxic effects in rats
Gomez, MR; Quiles, CH; Gutierrez, MG; Ocana, JG; Alcaraz, RP; Lara, VA; Leon, RG; Wittel, MB; Baturone, MO
Hip fracture co-management in the elderly in a tertiary referral hospital: A cohorts study
Portoles-Callejon, A; Lopez-Alfaro, R; Gimenez-Miranda, L; Lopez-Rios, MC; Varela-Aguilar, JM; Calderon, E; Medrano, FJ
Adequacy and prognostic impact of treatment for severe exacerbation of chronic obstructive pulmonary disease
Garcia, C; Ochoa, T; Neyra, E; Bustamante, B; Ponce, C; Calderon, EJ; Vargas, SL
Pneumocystis jirovecii colonisation in pregnant women and newborns in Lima, Peru
de Miguel-Diez, J; Blasco-Esquivias, I; Rodriguez-Matute, C; Bedate-Diaz, P; Lopez-Reyes, R; Fernandez-Capitan, C; Garcia-Fuika, S; Lobo-Beristain, JL; Garcia-Lozaga, A; Quezada, CA; Murga-Arizabaleta, I; Garcia-Ortega, A; Rodriguez-Davila, MA; Marin-Barrera, L; Otero-Candelera, R; Praena-Fernandez, JM; Jara-Palomares, L
Correlation between short-term air pollution exposure and unprovoked lung embolism. Prospective observational (Contamina-TEP Group)
Penalva, G; Fernandez-Urrusuno, R; Turmo, JM; Hernandez-Soto, R; Pajares, I; Carrion, L; Vazquez-Cruz, I; Botello, B; Garcia-Robredo, B; Camara-Mestres, M; Dominguez-Camacho, JC; Aguilar-Carnerero, MM; Lepe, JA; de Cueto, M; Serrano-Martino, MC; Dominguez-Jimenez, MC; Dominguez-Castano, A; Cisneros, JM; Alcantara, JD; Marin, RA; Vega, AD; de la Cuesta, JD; Fajardo, JM; Moreno, JG; Hevia, E; Irastorza, A; Vazquez, AM; Molina, J; Montero, E; Aguilar, JLP; Remesal, T; Retamar, P; Prieto, ST; Toral, J; Moreno, JT
Long-term impact of an educational antimicrobial stewardship programme in primary care on infections caused by extended-spectrum beta-lactamase-producing Escherichia coli in the community: an interrupted time-series analysis
de Armas, Y; Capo, V; Bornay-Linares, FJ; del Aguila, C; Matos, O; Calderon, EJ
Pneumocystis jirovecii and microsporidia: An unusual coinfection in HIV patients?
Leon-Jimenez, D; Lopez-Mendoza, M; Perez-Temprano, R; Gorriz, JL; Miramontes-Gonzalez, JP
In response to the paper 'High-protein diet: A barrier to the nephroprotective effects of sodium-glucose co-transporter-2 inhibitors?'
de Miguel, PA; Enguix-Riego, MV; Cacicedo, J; Delgado, BD; Perez, M; Praena-Fernandez, JM; Cortes, LQ; Garcia, PB; del Campo, ER; Guerra, JLL
Prognostic value of the TGF beta 1 rs4803455 single nucleotide polymorphism in small cell lung cancer
Mate, A; Blanca, AJ; Salsoso, R; Toledo, F; Stiefel, P; Sobrevia, L; Vazquez, CM
Response to Letter to the Editor by Briana and Malamitsi-Puchner: Effects of Pregnancy-induced Insulin Resistance on the Fetus and the Future Development of Metabolic Diseases in Adulthood
Santana-Garrido, A; Reyes-Goya, C; Perez-Camino, MC; Andre, H; Mate, A; Vazquez, CM
Retinoprotective Effect of Wild Olive (Acebuche) Oil-Enriched Diet against Ocular Oxidative Stress Induced by Arterial Hypertension
Penate, X; Praena-Fernandez, JM; Pareja, PR; Enguix-Riego, MD; Payan-Bravo, L; Vieites, B; Gomez-Izquierdo, L; Olasolo, JJ; del Campo, ER; Reyes, JC; Chavez, S; Lopez Guerra, JL
Overexpression of Canonical Prefoldin Associates with the Risk of Mortality and Metastasis in Non-Small Cell Lung Cancer
Reyes-Goya, C; Santana-Garrido, A; Soto-Astacio, E; Aramburu, O; Zambrano, S; Mate, A; Vazquez, CM
Mechanism of Vascular Toxicity in Rats Subjected to Treatment with a Tyrosine Kinase Inhibitor
Guisado-Gil, AB; Munoz-Burgos, M; Ortega-Eslava, A; Garcia-Hernandez, FJ; Santos-Ramos, B
Cost-minimization analysis of individually tailored or fixed-scheduled rituximab maintenance therapy for antineutrophil-cytoplasm antibody associated vasculitides
Pla, L; Avino, A; Eritja, R; Ruiz-Gaitan, A; Peman, J; Friaza, V; Calderon, EJ; Aznar, E; Martinez-Manez, R; Santiago-Felipe, S
Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples
Calvo-Lozano, O; Avino, A; Friaza, V; Medina-Escuela, A; Huertas, CS; Calderon, EJ; Eritja, R; Lechuga, LM
Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
Alonso R, Pérez de Isla L, Múñiz-Grijalvo O, Díaz-Díaz JL, Mata P.
Familial Hypercholesterolaemia Diagnosis and Management.
Eur Cardiol.
Arévalo-Lorido JC, Gómez JC, Huelgas RG, de Lucas DG, Polo LM, Aguilar JMV, Muñoz JE.
Lowering Blood Pressure with the Combination of a Sodium-Glucose Cotransporter 2 Inhibitor and a Glucagon-like Peptide-1 Receptor Agonist in Type 2 Diabetic Patients: A Clinical Evidence.
High Blood Press Cardiovasc Prev
Ariza MJ, Rioja J, Ibarretxe D, Camacho A, Díaz-Díaz JL, Mangas A, Carbayo-Herencia JA, Ruiz-Ocaña P, Lamíquiz-Moneo I, Mosquera D, Sáenz P, Masana L, Múñiz-Grijalvo O, Pérez-Calahorra S, Valdivielso P, Spanish Dyslipidemia Registry.
Molecular basis of the familial chylomicronemia syndrome in patients from the National Dyslipidemia Registry of the Spanish Atherosclerosis Society.
J Clin Lipidol.
Avendaño MS, García-Redondo AB, Zalba G, González-Amor M, Aguado A, Martínez-Revelles S, Beltrán LM, Camacho M, Cachofeiro V, Alonso MJ, Salaices M, Briones AM.
mPGES-1 (Microsomal Prostaglandin E Synthase-1) Mediates Vascular Dysfunction in Hypertension Through Oxidative Stress.
Blom DJ, O'Dea L, Digenio A, Alexander VJ, Karwatowska-Prokopczuk E, Williams KR, Hemphill L, Múñiz-Grijalvo O, Santos RD, Baum S, Witztum JL.
Characterizing familial chylomicronemia syndrome: Baseline data of the APPROACH study.
J Clin Lipidol
Calero Bernal ML, Varela Aguilar JM.
Infant-juvenile type 2 diabetes.
Rev Clin Esp
Carretero Gómez J, Miramontes González JP, Varela Aguilar JM, Ena J, Arévalo Lorido JC, Diabetes and Obesity Working Group of Spanish Society of Internal Medicine.
Use of degludec insulin in chronic complex patients
Eur J Intern Med.
Delaye L, Ruiz-Ruiz S, Calderón E, Tarazona S, Conesa A, Moya A.
Evidence of the Red-Queen Hypothesis from Accelerated Rates of Evolution of Genes Involved in Biotic Interactions in Pneumocystis
Genome Biol Evol.
González-Echavarri C, Capdevila O, Espinosa G, Suárez S, Marín-Ballvé A, González-León R, Rodríguez-Carballeira M, Fonseca-Aizpuru E, Pinilla B, Pallarés L, Ruiz-Irastorza G, RELES, Autoimmune Diseases Study Group GEAS.
Infections in newly diagnosed Spanish patients with systemic lupus erythematosus: data from the RELES cohort.
León Jiménez D, Castilla Guerra L, López Chozas JM, Miramontes González JP.
Update concept of the dual blocking of the renin angiotensin aldosteron system. A new therapeutic option?
Med Clin (Barc).
Ruiz-Ruiz F, Medrano FJ, Navarro-Puerto MA, Rodríguez-Torres P, Romero-Alonso A, Santos-Lozano JM, Alonso-Ortiz Del Rio C, Varela-Aguilar JM, Calderón EJ, Marín-León I; en nombre de los integrantes del grupo de trabajo INTROMBIN.
Delphi-RAND consensus of the Spanish Society of Internal Medicine on the controversies in anticoagulant therapy and prophylaxis in medical diseases. INTROMBIN Project (Uncertainty in thromboprophylaxis in internal medicine).
Rev Clin Esp.
Martínez-Rísquez MT, Friaza V, de la Horra C, Martín-Juan J, Calderón EJ, Medrano FJ.
Pneumocystis jirovecii infection in patients with acute interstitial pneumonia.
Rev Clin Esp
. Madrid L, Varo R, Maculuve S, Nhampossa T, Muñoz-Almagro C, Calderón EJ, Esteva C, Carrilho C, Ismail M, Vieites B, Friaza V, Lozano-Dominguez MDC, Menéndez C, Bassat Q.
Congenital cytomegalovirus, parvovirus and enterovirus infection in Mozambican newborns at birth: A cross-sectional survey
PLoS One.
Nevez G, Robert-Gangneux F, Pougnet L, Virmaux M, Belleguic C, Deneuville E, Rault G, Chevrier S, Ramel S, Le Bihan J, Guillaud-Saumur T, Calderon E, Le Govic Y, Gangneux JP, Le Gal S.
Pneumocystis jirovecii and Cystic Fibrosis in Brittany, France.
Spyropoulos AC, Ageno W, Albers GW, Elliott CG, Halperin JL, Hiatt WR, Maynard GA, Steg PG, Weitz JI, Suh E, Spiro TE, Barnathan ES, Raskob GE; MARINER Investigators.
Rivaroxaban for Thromboprophylaxis after Hospitalization for Medical Illness
N Engl J Med.